Mcf10at1

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August undefined, 2024

WebFor these studies, we used MCF10AT1 cells, a model for progressive breast cancer, injected into the mammary fatpad of nude mice as a single cell suspension. The mice were fed 0.1% sphingomyelin supplements in a semi-purified AIN76A control diet when the lesions were palpable. Web19 jul. 2000 · Ductal carcinoma in situ (DCIS) is becoming increasingly common, accounting for 25%–30% of newly diagnosed cases of breast cancer (). The comedo type represents …

Role of Secreted Frizzled-Related Protein 1 in Early Breast ...

Web3 jun. 2016 · The MCF10AT1 tumors and mammary fat pads were paraffin embedded and sectioned at the Microscopy and Histology Facility, PSU College of Medicine, Hershey, … Web1 okt. 2011 · Here we show that the AHR was functionally activated when estrogen receptor-negative, AHR overexpressing MCF10AT1 human breast cancer cells (designated … target ceramic love bird

Metabotropic Glutamate Receptor-1 Contributes to Progression in …

WebPlus, since this subline showed a similar increase in ESR1 expression as the other MCF10AT1 and the MCF10A sublines, cell viability cannot be linked to the increased ESR1 expression. It seems to me that even the 2-3-fold increase of the low ESR1 expression does not make viability of MCF10A cells more dependent on ESR1. Webformation, MCF10AT1 were embedded into 6 mg mL-1 collagen using a custom-made device for improved confocal imaging (Figure 1c). This collagen concentration yielded hydrogels with an elastic modulus of 2214.5 +/– 218 Pa mimicking the mechanical properties of collagen-rich interstitial ECM of obese breast tissue (Figure 1d). WebCRL-10317 ™. MCF 10A is an epithelial cell line that was isolated in 1984 from the mammary gland of a White, 36-year-old female with fibrocystic breasts. This cell line was … target ceramic serving bowl

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Methionine-restricted diet inhibits growth of MCF10AT1 …

WebMethods: Athymic nude mice were injected with MCF10AT1 cells in Matrigel® and fed a diet containing either 0. 86 % methionine (control fed, CF), or 0.12 % methionine (MR) for 12 weeks. Plasma amino acid concentrations were measured by UPLC, and proliferation and apoptosis were examined using RT-PCR, immunohistochemistry, and Cell Web24 mrt. 2009 · The MCF10AT1K.cl2 variant retained an MCF10AT1-type pattern of xenograft growth but also displayed chromosomal aberrations more characteristic of … target chadstone shopping centreWeb17 jul. 2007 · MCF10AT1 cells were exposed for 20 passages with beta-HCH, 4-OH-Tamoxifen (Tam), or 17-beta-estradiol (E2) after which cells were analyzed for … target ceramic infrared bulb

"WebWe developed a model system to mechanistically examine BCSCs in basal-like breast cancer using MCF10AT1 FACS sorted for CD24 (negative/low in BCSCs) and CD44 (positive/high in BCSCs). Ingenuity Pathway Analysis comparing RNA-seq on the CD24 −/low versus CD24 +/high MCF10AT1 indicates that the top activated upstream … " - Mcf10at1

Mcf10at1

Ligand-independent activation of the arylhydrocarbon receptor

WebCYP1A1 is amongst the canonical genes regulated by AhR with a promoter containing typical XRE, we further investigated endogenous CYP1A1 mRNA levels in MCF10AT1 … Web24 mrt. 2009 · However, MCF10AT1 cells form lesions consisting only of simple ducts in ovariectomized nude mice , which have serum E2 levels of ~5 pg/ml . Therefore, MCF10AT1 xenografts respond to very low doses of E2 in vivo, suggesting that the levels of estrogen-processing enzymes in breast cells may play a critical role in determining growth response.

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Web17 jul. 2007 · In our in vivo studies, MMTV-Neu mice were injected with β-HCH and observed for tumor formation over a 70 week period. β-HCH and Tam selected MCF10AT1 cells demonstrated increased mRNA expression of MMP-13 (collagenase-3) a marker of increased invasiveness. β-HCH treatment was also seen to increase the expression in a … Web2 aug. 2024 · MCF10AT1-CRYβB2 tumors were less differentiated and resembled squamous cell carcinoma, while MCF10AT1-vector tumors were more differentiated and predominantly expressed features of adenocarcinoma ...

WebIn MCF10AT1 cells, the MAPK pathway is rapidly activated (increase in the phospho-p42/p44 MAPK/MAPK ratio) in the presence of the selective GPR30 agonist G1 (66) and … Web1 jan. 1996 · By reestablishing cells in tissue culture from one of the carcinomas, a cell line designated MCF10AT1 was derived that forms simple ducts when transplanted in …

WebNational Center for Biotechnology Information Web14 jul. 2016 · Hens JR, Sinha I, Perodin F, Cooper T, Sinha R, Plummer J, et al. Methionine-restricted diet inhibits growth of MCF10AT1-derived mammary tumors by increasing cell …

Web13 jan. 2024 · It is also unclear how common genetic defects, such as p53 mutations, modify these stromal-epithelial interactions. To address these knowledge gaps, we leveraged the MCF10 progression series of breast cell lines (MCF10A, MCF10AT1, and MCF10DCIS) to develop a longitudinal, tissue-contextualized model of p53-deficient, pre-malignant breast.

Web15 jul. 2009 · Four marker chromosomes were identified in MCF10A and MCF10AT1 and nine in the malignant MCF10CA1a cell line (Supplementary Table S3). MCF10A showed the characteristic (3;9) reciprocal translocation that has been described as the single most important event in the immortalization and transformation of the MCF10 breast epithelial … target cereal bowls stonewareWeb1 aug. 2009 · MCF10A (also referred as M1), a benign epithelial human cell line, and its metastatic mutant cell line counterpart MCF10CA1 (also referred as M4), were studied … target ceramic pots and pansWebMCF10AT1 cells, wild type or transduced with either LacZ or GRM1, were implanted into both flanks of athymic nude mice and allowed to grow for 8 weeks, after which … target chairs accentWeb8 sep. 2016 · Tumor development and tumor progression is not only determined by the corresponding tumor cells but also by the tumor microenvironment. This includes an orchestrated network of interacting cell types (e.g. immune cells, endothelial cells, fibroblasts, and mesenchymal stroma/stem cells (MSC)) via the extracellular matrix and … target ceramic table christmas treeWebHere we show that the AHR was functionally activated when estrogen receptor-negative, AHR overexpressing MCF10AT1 human breast cancer cells (designated P20E) were … target ceramic watering canWeb13 mei 2015 · These cell lines include MCF10AT1 (MCF10AT), a premalignant cell line derived from MCF10A transfected with H-Ras , and a set of oncogenic MCF10CA cell … target cereal shelfNational Center for Biotechnology Information target ceramic tall mug