Cisplatin hela

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August undefined, 2024

WebFor IFNβ-cisplatin sequential treatment protocol (E), HeLa cells were treated with 100 ng/mL IFNβ for 24, 48, or 72 h, and then cells were replated in 96-well plates and treated … WebThe cis-Diammineplatinum (II) dichloride (cisplatin; P4394), 3- (4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT; M2128), and dimethyl sulfoxide (DMSO; D2650 for cell stock storage and D5979 for drug preparation) were purchased from Sigma (St. Louis, MO, USA).

CaSki, SiHa, HeLa, and C33A cervical cancer cells display …

WebIn order to assist in optimizing treatment, we applied 5-ALA/PDT in combination with low-dose cisplatin to evaluate cytotoxicity in Hela cells. Methods: Antiproliferative effects of 5-ALA/PDT and cisplatin, alone and in combination, were assessed using MTT assay. WebCisplatin, as a significant chemotherapeutic drug for the treatment of cancers, was combined with rapamycin (RAPA), an autophagy inducer, or 3-methyladenine (3-MA), an autophagy inhibitor, and these cisplatin … how much money do i take home after taxes

IJMS Free Full-Text NHERF1 Enhances Cisplatin Sensitivity in …

WebJun 1, 2024 · IC90 of paclitaxel in HeLa Pac RB was increased from 69 pM, 440 pM, 2,561 pM and 10,337 pM on 0th, 1st, 2nd, 3rd and 4th months, respectively. Interestingly, the resistant cells were recovered to... WebNov 29, 2024 · Cisplatin-resistant A549 non-small cell lung cancer cells can be identified by increased mitochondrial mass and are sensitive to pemetrexed treatment Cisplatin-resistant A549 non-small cell lung cancer cells can be identified by increased mitochondrial mass and are sensitive to pemetrexed treatment WebJan 12, 2024 · Cisplatin-resistant HeLa cells were transfected with NHERF1 and overexpression of NHERF1 protein was confirmed by Western blotting ( Figure 2 A). Effect of NHERF1 on cell growth was examined by CCK8 … how much money do i spend

Cancers Free Full-Text PARP1-Inhibition Sensitizes Cervical …

WebJul 22, 2024 · In contrast, artesunate preferably accumulates in lysosomes, activates lysosome function, and promotes ferritin degradation and free ferrous (Fe 2+) release in HeLa cervical cancer cells . This artesunate-mediated effect on the lysosome and cellular iron accumulation may also occur in HCC cells, and artesunate may further synergize … WebFeb 12, 2024 · MBNL1 regulates resistance of HeLa cells to cisplatin via Nrf2 Chemotherapy is an important method in the treatment of cervical cancer, but some patients will face drug resistance, which often indicates a poor prognosis. Moreover, there is no complete solution at present. how do i play from usb on slingboxWebAug 6, 2024 · Cisplatin is a platinum-based chemotherapeutic agent that is widely used for the treatment of malignant tumors, such as cervical cancer, lung cancer, and ovarian cancer [3]. Cisplatin mainly induces cross-linking at the N7-position of guanosine, which modifies DNA to induce apoptosis and kill cancer cells [4]. how do i play farmville on facebook

"WebSep 18, 2024 · National Center for Biotechnology Information " - Cisplatin hela

Cisplatin hela

Phospholipase A2-activating protein (PLAA) enhances cisplatin …

WebThe chemotherapeutic agent cisplatin, which is a small-molecule platinum compound and was originally found to inhibit bacterial growth and later identified as an anticancer agent, appears to most effectively treat … WebSep 10, 2010 · Cisplatin has been widely accepted as one of the most efficient anticancer drugs for decades. However, the mechanisms for the cytotoxic effects of cisplatin are still not fully understood. Cisplatin primarily targets DNA, resulting in the formation of DNA double strand breaks and eventually causing cell death.

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WebOct 1, 2024 · The present study using network pharmacology revealed that salicylic acid and cisplatin shared common genes related to apoptosis in cervical cancer. Salicylic acid … WebFeb 28, 2024 · One of the main problems in chemotherapy using platinum drugs as anticancer agents is the resistance phenomenon. Synthesizing and evaluating valid alternative compounds is challenging. This review focuses on the last two years of progress in the studies of platinum (II)- and platinum (IV)-based anticancer complexes. In …

WebMay 1, 1996 · The intraperitoneal (i.p.) administration of cisplatin (CDDP) is one of the most effective therapies for cancers that are confined to the abdominal cavity. However, the … WebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, small cell lung cancer, germ cell tumors, lymphoma, and ovarian cancer. While it is often …

WebFeb 1, 2015 · In cell proliferation experiments, HeLa cells were treated with either cisplatin or myricetin and methyl eugenol alone, or vehiclealone for 12, 24 and 48 h. For apoptosis assay, cells weretreated with cisplatin or myricetin and methyl eugenol either alone or combined, or vehicle alone for 48 h. WebDrug: Cisplatin - Cancerrxgene - Genomics of Drug Sensitivity in Cancer Genomics of Drug Sensitivity in Cancer Compound: Cisplatin Drug Target: DNA crosslinker Drug Target …

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WebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa … how do i play gta rp serversWebJul 1, 2009 · To test our hypothesis, we employed HeLa Tet (tetracycline)-off cells, which we previously used to overexpress the native plaa gene [21], and determined their apoptotic responses to cisplatin. HeLa cells were chosen because cervical tumor is a cisplatin-responsive tumor, while cervical tissues express the plaa gene [31], and respond to ... how do i play draughtsWebJan 20, 2024 · Further investigation revealed that cisplatin-resistant cells sustain OXPHOS partly via iron metabolism reprogramming during glutamine deprivation. This reprogramming might be responsible for mitochondrial iron-sulfur [Fe-S] cluster biogenesis, which has become an "Achilles' heel," rendering cancer cells vulnerable to DFO-induced autophagic ... how do i play gin rummyWebIn vitro response to ionizing radiation (IR), cisplatin (cDDP), hyperthermia (HT) and PARP1-inhibition (PARP1- i) as single and combinational treatments in four cervical cancer cell lines (SiHa, HeLa, CaSki and C33A). ( A) Normalized colony formation after IR in combination with cDDP or ( B) with HT. how much money do i need to put down on houseWebFeb 11, 2024 · Subsequently, cells were treated with IC 50 doses of 5-FU (17.58 μM: SiHa; 15.31 μM: HeLa) and cisplatin (24.14 μM: SiHa; 6.82 μM: HeLa) for 48 h and the exosomes were isolated from the conditioned media of treated cells. The exosomal RNA was isolated and examined for modulation of HPV transcripts by type-specific HPVE6 and E7 RT-PCR. how do i play fortnite on xbox cloud gaming how do i play gin rummy with 2 playersWebCisplatin combination drugs induce stronger effects on autophagy than either of the components does. Combination drug-induced autophagy inhibits the growth of HeLa cell in a dose-dependent manner and … how much money do i take home