Cisplatin dna cross-linking

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August undefined, 2024

WebTumour cells isolated from 50 ovarian cancer patients were treated ex vivo with 100 μ M cisplatin for 1 h and crosslink formation and repair (unhooking) measured. ... in cellular mechanisms such as drug transport or detoxification. In contrast, the percentage repair (unhooking) of DNA interstrand crosslinks was much greater in the group of ... WebCisplatin induces DNA damage through inter-strand or intra-strand cross-linking of DNA . Its dose-limiting toxicity is nephrotoxicity, peripheral nerve toxicity, and toxicity to the cochlea (the inner ear toxicity) . Paclitaxel is a microtubule poison that …

Mechanism of the formation of DNA–protein cross-links …

WebApr 17, 2024 · The antitumor effects of cisplatin and other platinum compounds are attributed to their ability to induce interstrand DNA-DNA cross-links, which are thought … WebDNA interstrand crosslinks (ICLs) formed by antitumor agents, such as cisplatin or mitomycin C, are highly cytotoxic DNA lesions. Their repair is believed to be triggered primarily by the stalling of replication forks at ICLs in S-phase. There is, however, increasing evidence that ICL repair can also occur independently of replication. cipher labs covid test

Theoretical Study of Cisplatin Binding to Purine Bases: Why Does ...

WebCisplatin crosslinks DNA in several different ways, interfering with cell division by mitosis. The damaged DNA elicits DNA repair mechanisms, which in turn activate apoptosis when repair proves impossible. WebThis chapter deals with the interstrand cross-links in cisplatin- or transplatin-modified DNA and focuses on three main aspects: the formation of the interstrand cross-links, the distortions induced in the DNA double helix and the potential use of the interstrand cross-linking reaction in the context of antisense and antigene strategies. WebThe DNA binding is similar to cisplatin, and the complex forms a highly stereoselective adduct on DNA, but not for reactions with mono- and dinucleotides. 98 In cell lines with previously determined mechanisms of cell resistance, AMD473 showed little cross-resistance compared with cisplatin or carboplatin. cipherlab scanner setup

Identification of genome-wide cisplatin cross-linking sites …

Differential expression of collateral sensitivity or resistance to ...

WebSep 28, 2012 · DNA cross-linking agents such as the nitrogen mustards (melphalan, chlorambucil, cyclophosphamide, ifosfamide), platinum drugs (cisplatin, carboplatin, oxaliplatin), chloroethylnitrosoureas (carmustine, lomustine), the alkylalkane sulphonate busulphan and the natural product mitomycin C are widely used drugs as both single … WebPhoto-cross-linking is thus an effective tool for investigating the interaction of cisplatin-modified DNA with damage-recognition proteins under heterogeneous conditions such those in cell extracts or living cells. † Supported by Grant … cipherlab singaporeWebDec 22, 2024 · Cisplatin has been widely used for the treatment of cancer and its antitumour activity is attributed to its capacity to form DNA adducts, predominantly at guanine residues, which impede cellular processes … dialyse bad windsheim fax

"WebOct 28, 2003 · A dominating preference for initial attack at the N7-position of guanine is established experimentally, which is a crucial first step for the formation of a 1,2-intrastrand cross-link of adjacent guanine bases that leads to bending and unwinding of DNA. " - Cisplatin dna cross-linking

Cisplatin dna cross-linking

The planar compound links to nucleobases through water displacement of one or both of its chloride groups, allowing cisplatin to form monoadducts to DNA or RNA, intrastrand DNA crosslinks, interstrand DNA crosslinks, and DNA-protein crosslinks. When cisplatin generates DNA crosslinks, it more frequently … See more In genetics, crosslinking of DNA occurs when various exogenous or endogenous agents react with two nucleotides of DNA, forming a covalent linkage between them. This crosslink can occur within the same strand (intrastrand) … See more Crosslinking of DNA and protein Biochemical interaction methods DNA-protein crosslinking can be caused by a variety of chemical and physical agents, including … See more Many characterized crosslinking agents have two independently reactive groups within the same molecule, each of which is able to bind with a nucleotide residue of DNA. These agents … See more Crosslinked DNA is repaired in cells by a combination of enzymes and other factors from the nucleotide excision repair (NER) pathway, See more • PDB: 1AIO – Interactive structure for cisplatin and DNA adduct formation • PDB: 204D – Interactive structure for psoralen and crosslinked DNA See more WebDNA crosslinking agents, such as cisplatin derivatives (carboplatin and oxaliplatin) and mitomycin C, are especially cytotoxic to tumor cells, and their therapeutic index derives, …

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WebCisplatin is one of the most widely used agents in cancer chemotherapy. Its mode of action is cross-linking of the DNA, which can kill cells. But which part of the genome is more … WebWe evaluated the growth and molecular interactions of the anti-EGFR antibody cetuximab (erbitux) and the DNA cross-linking agent cisplatin (cis-diammedichloroplatinum; CDDP) in the gefitinib-resistant MDA-MB-468 breast cancer cell line, an in vitro model system that shows many of the recurrent basal-like molecular abnormalities including …

WebMar 15, 2007 · DNA–protein cross-linking by cisplatin or transplatin. Cisplatin and transplatin were investigated for their ability to form ternary DNA–protein complexes … WebFeb 1, 2002 · To overcome this obstacle, researchers have focussed their attention on cisplatin (cis-DDP; cis-platinum (II)diamminedichloride), a cross-linking agent shown to crosslink protein to DNA and...

WebJul 1, 2002 · Antitumor cisplatin [ cis -diamminedichloroplatinum (II)] forms on DNA predominantly intrastrand cross-links between neighboring purine residues. Several discoveries suggested that the toxicity of cisplatin originated from these lesions. WebDec 15, 1995 · The widely used antitumor drug cis -diamminedichloroplatinum (II) (cisplatin or cis -DDP) reacts with DNA, cross-linking two purine residues through the N7 …

WebJan 1, 2001 · We compared the DNA cross-linking capability of CC-1065 with that of its analogs: Bizelesin, which forms DNA cross-links by direct chemical bonding, and Adozelesin, a demethoxy derivative of CC-1065. Section snippets Drugs

Webfavors cisplatin cross-linking (20) with inter- and intra-DNA strands (14,17,26). Cisplatin interacts primarily with cytosine- and guanine-rich DNA regions (29) and causes cellular damage. Despite its potent antineoplastic action, cisplatin’s major side effects include nephrotoxicity (2), peripheral neuropathy (33), azoospermia (11). In mammals, dialyse bad windsheimWebFeb 28, 2024 · Indeed, while cisplatin probably undergoes an efflux effect, compound 19 shows a time-dependent accumulation in cells and a greater ability to cross the membrane. DNA interaction has been confirmed for both complexes due to the presence of the triphenylphosphine hydrophobic moiety and the trans geometry of bromide groups. dialyse bensheim nephrocareWebPhoto-cross-linking is thus an effective tool for investigating the interaction of cisplatin-modified DNA with damage-recognition proteins under heterogeneous conditions such … cipherlab south africaWebCisplatin produces DNA cross-linking and is used to treat medulloblastoma, head and neck, ovarian, germ cell, cervical, lung and bladder cancers. Cisplatin produces a … dialyse bayenthalWebAug 8, 2010 · Cisplatin-induced DNA damage activates various signaling pathways to prevent or promote cell death. This paper summarizes our current understandings … dialyse berlin buchWebJul 23, 1996 · Abstract cis-Diamminedichloroplatinum (II) (cisplatin) is a widely used anticancer drug that binds to and crosslinks DNA. The major DNA adduct of the drug results from coordination of two adjacent guanine bases to platinum to form the intrastrand crosslink cis- [Pt (NH3)2 [d (GpG)-N7 (1), -N7 (2)]] (cis-Pt-GG). dialyse besancon chuWebNov 15, 1987 · Two sublines were derived from a human bladder carcinoma continuous cell line (RT112-P), one by exposure to fractionated X-irradiation (RT112-DXR8) and the other by continuous exposure to cisplatin (RT112-CP). RT112-DXR8 cells were 1.6- to 2-fold more sensitive to cisplatin and 2 analogues, carbopla … dialyse bichat